Trial-unique Nonmatching-to-Location Task (TUNL) for Mice
Model 89545A
Adapted for mice, this is a working memory task. TUNL is a delayed nonmatching-to-place task that prevents the mediating strategies associated with the equivalent lever task.
TUNL can be thought of as a version of delayed nonmatching-to-place (DNMTP), in which the subjects are presented with a sample location, and following a delay, with the (incorrect, S-) sample location and a (correct, S+) nonmatching location. DNMTP has been shown to be vulnerable to non-spatial mediating strategies. TUNL eliminates these problems by using multiple, trial-unique locations, preventing the use of mediating strategies. Animals with lesions in the dorsal hippocampus or decreased hippocampal neurogenesis were impaired when the locations were close together, but not when they were far apart. This feature also renders the task exquisitely sensitive to hippocampal dysfunction, tapping both the role of the hippocampus in memory and in pattern separation. The rat version uses 15 individual locations. A simpler version using 5 positions is available for mice.
Example Neural Systems Involved
- Hippocampus
- Cholinergic system
- NMDA Receptors
- Prefrontal Cortex Amygdala
Clinical Areas Showing Impairment
- Schizophrenia
- Alzheimers's
Cognitive Functions Covered
- Working Memory
Typical Training Times (depends on strain and age)
- 1-2 weeks to train to touch the screen and initiate the task
- ~20 sessions
Sample Video
Rat
Rat performing the TUNL task with the 15 window mask.
Tethered Mouse
Tethered mouse performing the TUNL task with the 5 window mask, using the open reward trough 80614A-07-10.
Features
- A test of working memory
- All training paradigms and advice included
Science
Barnard, I. L., Onofrychuk, T. J., McElroy, D. L., & Howland, J. G. (2021). The Touchscreen‐Based Trial‐Unique, Nonmatching‐To‐Location (TUNL) Task as a Measure of Working Memory and Pattern Separation in Rats and Mice. Current Protocols, 1(9). - Abstract
Palmer, D., Dumont, J. R., Dexter, T. D., Prado, M. A. M., Finger, E., Bussey, T. J., & Saksida, L. M. (2021). Touchscreen cognitive testing: Cross-species translation and co-clinical trials in neurodegenerative and neuropsychiatric disease. In Neurobiology of Learning and Memory (Vol. 182, p. 107443). - Abstract
Shepherd, A., Zhang, T., Hoffmann, L. B., Zeleznikow-Johnston, A. M., Churilov, L., Hannan, A. J., & Burrows, E. L. (2021). A Preclinical Model of Computerized Cognitive Training: Touchscreen Cognitive Testing Enhances Cognition and Hippocampal Cellular Plasticity in Wildtype and Alzheimer's Disease Mice. Frontiers in Behavioral Neuroscience, 15(December), 1-15. - Abstract
Lee, J., van den Buuse, M., Nithianantharajah, J., & Jones, N. C. (2020). Acute NMDA receptor antagonism impairs working memory performance but not attention in rats-Implications for the NMDAr hypofunction theory of schizophrenia. Behavioral Neuroscience, 134(4), 323-331. - Abstract
Mosser, C.-A., Haqqee, Z., Nieto-Posadas, A., Murai, K., Stefano, S., Williams, S., & Brandon, M. P. (2020). The McGill-Mouse-Marmoset Platform: A Standardized Approach for High-throughput Imaging of Neuronal Dynamics During Behavior. BioRxiv. - Abstract
