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Quad channel Acrylic chamber system for Electrophysiology with heater & thermistor feedback control
Model 7450-4A-E
The new all-in-one chamber design incorporates the best features of both the old Haas or interface type cell and the old Oslo or submerged type cell.
This quad cell slice chamber is designed to work in either Submerged or Interface modes. The Model CI.7450-4A(E) features four acrylic tissue cell bodies, each with an Ag/AgCl reference electrode for tissue electrophysiology and four holding cells designed to keep additional slices viable until used. The unit could of course be used for biochemistry as well.
Substitute the Model CI.7450-4P(E) for cell bodies made of Teflon®. This is recommended in settings where pharmacological agents could be absorbed and later released by the acrylic cell body.
Stable, linear flow and temperature are assured in either Submerged or Interface mode. Replacement cell chambers are available in both Acrylic and P.T.F.E. (Teflon®) materials.
A digital display heater / controller unit is included with predictive algorithms (Proportional, Integral and Derivative) and dual feedback from a thermistor for fast feedback, plus another for spot temperature offset. The water bath temperature is controlled to within +/- 0.1 Celsius in a stable environment.
The ergonomics of being able to easily work with multiple slices has been carefully considered in the design of this unit and all multi-chamber systems. Tissue cells are spaced for micromanipulators to hold stimulator and recording electrodes on either side. The 4 channel chambers are the most cost and time efficient. They may be used alone or they can be built up into our semi-automated Workstation or fully automated Synchroslice integrated lab used for hippocampal and heart slice electrophysiology. With optional micromanipulators and overhead cameras, placement of 8 electrodes can be done within 20-30 minutes, with all slices viewed though a quad processor and video monitor.
Note: Single, dual and quad channel units are available with or without camera observation units and other accessories.
Features
- P.I.D. algorithm temperature controller with D.C power and very low noise output
- Stable temperature with two calibrated thermistors for feedback and offset
- Each Cell includes fluid level adjustment and a clear cover to maintain the critical environment for operating in either the Submerged (Oslo type) or Interface (Haas type) mode.
- A bubble escape in the perfusion line prevents disturbance of the slice on the net and so eliminates any related noise artifact.
- Easy to clean with low cost replacement chambers, tubing and reference electrodes
- Menus include alarms to monitor ambient temperature changes
- Add illumination into chamber to backlight for electrode placement
- Bubbling of water bath keeps dissolved oxygen pressure high
- A separate holding chamber keeps slices in stable rest for transfer to recording chamber
- Easy electrode placement using multi-channel CCD cameras and monoscope systems for monitoring all chambers
- Stable flow rates using 745-PP Smoothflow multi-channel pumps
